Synthesis of rhamnosylated arginine glycopeptides and determination of the glycosidic linkage in bacterial elongation factor P.

Synthesis of rhamnosylated arginine glycopeptides and determination of the glycosidic linkage in bacterial elongation factor P† †Electronic supplementary information (ESI) available: Materials and methods, compound characterization, 1D and 2D NMR and mass spectrometry data of glycopeptides. See DOI: 10.1039/c6sc03847f Click here for additional data file.

Resolving the A-glycosidic Linkage of Arginine- Rhamnosylated Translation Elongation Factor P Triggers Generation of the First Arg Rha Specific Antibody †

Department of Organic Chemistry, School University, Shanghai 200433, China. E-mai Department of Biology I, Microbiology, Lu Munich, Germany Center for Integrated Protein Science M München, Munich, Germany. E-mail: juerge Structural and Computational Biology Un Germany School of Pharmacy, Wei Fang Medical Uni Institute of Infection and Immunity, Taihe Shiyan, Hubei 442000, China † Electronic sup...

Resolving the α-glycosidic linkage of arginine-rhamnosylated translation elongation factor P triggers generation of the first ArgRha specific antibody† †Electronic supplementary information (ESI) available: Experimental details and chemical compound characterization. CCDC 1469830. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c6sc02889f Click here for additional data file. Click here for additional data file.

a Department of Organic Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, China b Department of Biology I, Microbiology, Ludwig Maximilians-Universität München, Munich, Germany Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universität München, Munich, Germany c Structural and Computational Biology Unit, EMBL Heidelberg, Heidelberg 69117, Germ...

Structural Basis for EarP-Mediated Arginine Glycosylation of Translation Elongation Factor EF-P

Glycosylation is a universal strategy to posttranslationally modify proteins. The recently discovered arginine rhamnosylation activates the polyproline-specific bacterial translation elongation factor EF-P. EF-P is rhamnosylated on arginine 32 by the glycosyltransferase EarP. However, the enzymatic mechanism remains elusive. In the present study, we solved the crystal structure of EarP from Pse...

Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B

Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococc...